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Part:BBa_K194003

Designed by: Christian Kaas   Group: iGEM09_DTU_Denmark   (2009-10-14)

USER cassette for insertion of USER fragments This biobrick will allow insertion of PCR fragments using the novel USERTM biobrick assembly standard (BBF RFC 39). It contains a PacI/Nt.BbvCI USER cassette for insertion of PCR fragments using the USER cloning standard. PCR fragments containing uracil is treated with a uracil DNA glycosylase that removes the uracil exposing a predesigned 8bp overhang allowing for cloning without the need for ligase. For more information concerning USER cloning see our wiki: http://2009.igem.org/Team:DTU_Denmark/USERprinciple

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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